TABLE 2

Real-time PCR confirmation of Leishmania donovani in infected mouse spleen tissues for treated and untreated groupsa

Group and serial no.DescriptionCT value
Treated groups
    1Naive mice31.859 ± 0.24
    2Vehicular control group14.799 ± 0.02
    3Miltefosine treatment23.160 ± 0.08
    4AN2690 treatment19.621 ± 0.88
Controls
    1Nontemplate control30.953 ± 0.94
    2Positive control (10 ng DNA from L. donovani parasites)12.492 ± 0.32
  • a The data presented are representative of those from two experiments with similar results and represent the mean ± SEM for 5 mice in each group.