TABLE 4

Primers used for molecular characterization of the genetically manipulated parasites by PCR-based analysis

PrimerSequence
15′-TGTAGAAGTACTCGCCGATAGTGG-3′
25′-GTTTCAGGTGGCACCAGAAGA-3′
35′-CGCAGCTATTTACCCGCAGGACAT-3′
45′-TGGCCTTTAGCGTCTTCAGCGT-3′
55′-ATAGCGTTGGCTACCCGTGATATTGC-3′
65′-AACACGGCGGCATCAGAGCAGCCGATTG-3′
75′-TCATCGACGATGCCGTGATGTT-3′
85′-TACATGAACGGCAAGCTTCACCT-3′