TABLE 1

Antimicrobial susceptibility of CT-Eco2 and E. coli BL21(DE3) with and without blaTEM-184 gene

SpeciesIsolate nameGenotypeMIC (mg/liter)a
CTXCAZFEPAZTCAZ-AVIAZT-AVIPIPIMIMEMERTAKGMCIPPTZ
E. coliCT-Eco2CTX-M-14+TEM-18464>128>32>64≤1b>1280.50.250.1210.5>2>256
E. coliBL21(DE3)pLB-II-TEM-18411284640.250.12>1280.120.250.1210.50.120.5
E. coliBL21(DE3)pLB-II≤0.06≤0.06≤0.060.5≤0.060.50.50.120.120.1210.120.120.5
  • a MICs were determined using reference broth microdilution (17). Category classification was based on EUCAST clinical breakpoints (http://www.eucast.org/clinical_breakpoints). CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; CAZ-AVI, ceftazidime-avibactam (avibactam at fixed concentration of 4 mg/liter); AZT-AVI, aztreonam-avibactam (avibactam at fixed concentration of 4 mg/liter); PIP, piperacillin; IMI, imipenem; MEM, meropenem; ERT, ertapenem; AK, amikacin; GM, gentamicin; CIP, ciprofloxacin; PTZ, piperacillin-tazobactam (tazobactam at fixed concentration of 4 mg/liter).

  • b Not tested.