TABLE 3

Primers used for generation of hygromycin- and neomycin-specific linear replacement cassette fragments

PrimeraSequence
A5′-TTGAAGGCTCACATGTCGCGTA-3′
BHYG5′-GGTGAGTTCAGGCTTTTTCATGTTCTTCTCCAGCTGCGCCTTCA-3′
BNEO5′-CAATCCATCTTGTTCAATCATGTTCTTCTCCAGCTGCGCCTTCA-3′
CHYG5′-TGAAGGCGCAGCTGGAGAAGAACATGAAAAAGCCTGAACTCACC-3′
CNEO5′-TGAAGGCGCAGCTGGAGAAGAACATGATTGAACAAGATGGATTG-3′
DHYG5′-GAAGAGGGTAGACACCCCAACGTCTATTCCTTTGCCCTCGGACGAG-3′
DNEO5′-GAAGAGGGTAGACACCCCAACGTTCAGAAGAACTCGTCAAGAAG-3′
EHYG5′-CTCGTCCGAGGGCAAAGGAATAGACGTTGGGGTGTCTACCCTCTTC-3′
ENEO5′-CTTCTTGACGAGTTCTTCTGAACGTTGGGGTGTCTACCCTCTTC-3′
F5′-ATGCTTGTCCCGTTCGTGTCGT-3′
G5′-TTTTTCTAGAATGTCCACGGCACGTCGCGATG-3′
H5′-TTTTTTCATATGCTACTCGCGCTTCACTACTGG-3′
  • a HYG, hygromycin; NEO, neomycin.