TABLE 2

MIC values for colistin and polymyxin against E. coli strain DH5α carrying the wild-type mcr-3 gene or either of the two mutation variants (Thr277Ala and Thr277Ser)

E. coli strainMIC (μg/ml)
ColistinPolymyxin
DH5α0.50.5
DH5α(pUC19)0.50.5
DH5α(pUC19-mcr-3)44
DH5α(pUC19-mcr-3) (Thr277Ala)0.50.5
DH5α(pUC19-mcr-3) (Thr277Ser)11