TABLE 1

Azole antifungal susceptibility data and substitutions in cyp51 and transporter genes in non-WT (n = 3) and WT (n = 8) Aspergillus flavus isolatesa

StrainMIC (μg/ml)Substitution(s) in:
ITCVRCPOScyp51 genesEfflux pump genes
cyp51Acyp51Bcyp51CMDR1MDR2mfs1atrF
VPCI 195/P/10120.125R450SNoS240A,b T788G,b D254N,b I285V,b S196F, A324P, N423D, V465MNoneQ377H, R578S, G566EA434SNone
VPCI 982/P/15>16>16>8A205T,b R450SNoneS240A, M54T,b T788GT120K, F1105LF162L, Q377H, R578SE7G, A434SNone
VPCI 985/P/150.2520.06A205T,b R450SQ354KS240A, D254N, M54T, T788G, I285VbNoneF162L, Q377H, R578SE7G, A434SNone
  • a Wild-type A. flavus (n = 8) MIC ranges: itraconazole (ITC), 0.06 to 0.25 μg/ml; voriconazole (VRC), 0.125 to 1 μg/ml; and posaconazole (POS), 0.06 to 0.25 μg/ml. The 4 novel substitutions studied by homology modeling are in bold.

  • b Substitutions also observed in wild-type strains (VRC MIC range, 0.125 to 0.25 μg/ml).