TABLE 1

Cyp51A modifications and geometric mean MICs and MIC ranges obtained by EUCAST antifungal susceptibility testing for the A. fumigatus clinical strains

StrainCyp51A amino acid change(s)aMICb/MIC range (μg/ml)Isolation yr
ITCVRCPOS
AF237WTc0.250/0.250.354/0.25–0.50.085/0.06–0.12
ΔakuBKU80WT0.175/0.06–0.250.297/0.25–0.50.036/0.03–0.06d
CBS144.89/A1163WT0.210/0.125–0.250.500/0.50.036/0.015–0.06
AF293F46Y, M172V, N248T, D255E, E427K0.354/0.25–0.51.000/1.00.085/0.06–0.12
TP11F46Y, M172V, N248T, D255E, E427K0.630/0.25–2.01.000/0.5–2.00.139/0.06–0.52001
TP12F46Y, M172V, N248T, D255E, E427K0.500/0.51.000/1.00.120/0.122001
CM7632F46Y, M172V, N248T, D255E, E427K0.794/0.5–1.01.000/1.00.095/0.122015
CM2495F46Y, M172V, E427K0.250/0.250.500/0.50.085/0.06–0.122002
CM2730F46Y, M172V, E427K0.354/0.25–0.50.707/0.5–1.00.060/0.062004
CM2733F46Y, M172V, E427K0.354/0.25–0.50.707/0.5–1.00.060/0.062004
CM3249F46Y, M172V, E427K0.707/0.5–1.01.000/0.5–2.00.188/0.06–0.252005
CM3262F46Y, M172V, E427K1.000/1.00.500/0.50.120/0.122005
CM4602F46Y, M172V, E427K0.250/0.250.500/0.50.060/0.062006
CM4946F46Y, M172V, E427K0.397/0.125–1.00.794/0.5–1.00.097/0.06–0.252007
TP32F46Y, M172V, E427K0.630/0.25–1.00.397/0.25–1.00.095/0.06–0.122010
CM7560F46Y, M172V, E427K0.707/0.5–1.00.707/0.5–1.00.122/0.06–0.252014
CM7570F46Y, M172V, E427K0.630/0.5–1.00.500/0.50.397/0.25–0.52014
CM8726F46Y, M172V, E427K1.000/1.01.000/1.00.250/0.252016
  • a The Cyp51A protein with GenBank accession number AKK73659 was used as a reference.

  • b MICs are the geometric mean MICs. ITC, itraconazole; VRC, voriconazole; POS, posaconazole. EUCAST susceptibility tests were repeated for each strain at least twice on different days.

  • c WT, wild type.

  • d —, see http://www.aspergillusgenome.org/.