TABLE 2

Geometric mean MICs and MIC range obtained by EUCAST antifungal susceptibility testing for the A. fumigatus mutants generated and their control strains

StrainCyp51A amino acid change(s)aMICb/MIC range (μg/ml)
AMBITCVRCPOS
ΔakuBKU80WTc0.177/0.125–0.250.175/0.06–0.50.297/0.25–0.50.036/0.03–0.06
TP11F46Y, M172V, N248T, D255E, E427K0.250/0.125–0.250.630/0.25–2.01.000/0.5–2.00.139/0.06–0.5
CM3249F46Y, M172V, N248T0.250/0.125–0.250.707/0.5–1.01.000/0.5–2.00.188/0.06–0.5
T1.15Δcyp51A (cyp51A knockout)0.250/0.250.038/0.03–0.060.087/0.06–0.1250.019/0.015–0.03
T1.5F46Y, M172V, E427K, D255E, E427K0.125/0.1250.250/0.250.500/0.50.042/0.03–0.06
T2.3F46Y, M172V, E427K0.125/0.1250.250/0.250.500/0.50.030/0.03
T3.1F46Y0.125/0.1250.125/0.1250.354/0.25–0.50.021/0.015–0.03
  • a The Cyp51A protein with GenBank accession number AKK73659 was used as a reference.

  • b MICs are the geometric mean MICs. AMB, amphotericin B; ITC, itraconazole; VRC, voriconazole; POS, posaconazole. Each assay was repeated in duplicate with RNA from two different extractions.

  • c WT, wild type.