TABLE 1

AT mutant lytic activity on rabbit RBC and human A549 cell linea

WT or mutantActivity for:
A549RBC
LD50 (μg/ml)P value (WT vs mutant)LD50 (ng/ml)P value (WT vs mutant)
WT5.1212.12
D183A12.9<0.0001*24.210.0357*
S186A6.50.403919.710.2361
W187A>20<0.0001*38.58<0.0001*
N188A>20<0.0001*48.87<0.0001*
P189A5.80.941311.14>0.9999
V190A12.9<0.0001*50.79<0.0001*
R200A>20<0.0001*197.4<0.0001*
T263A10.80.0112*13.40.997
K266A10<0.0001*13.610.9997
  • a Rabbit RBC or the A549 human cell line was incubated with AT at the doses indicated in Fig. S1. The 50% lethal dose (LD50), corresponding to the [AT] required for 50% lysis, was calculated. Statistical difference for LD50 between WT-AT and mutants was considered statistically significant at P < 0.05 and is indicated with an asterisk.