TABLE 2

Cytotoxicity and anti-HBV effect of NVR 3-778 in HBV-infected PHHs in the presence and absence of plasma proteinsa

ConditionCytotoxicity (CC50 [μM])bEC50 for extracellular HBV DNA (μM)cEC90 for extracellular HBV DNA (μM)c
Days 0–8Days 5–12Days 0–8Days 5–12Days 0–8Days 5–12
Mean (95% CI)Fold changeMean (95% CI)Fold changeMean (95% CI)Fold changeMean (95% CI)Fold changeMean (95% CI)Fold changeMean (95% CI)Fold change
no BSA, no hA1GP15.85 (14.32–17.55)>251.256 (0.792–1.945)0.906 (0.562–1.338)4.660 (2.356–15.740)4.267 (2.142–13.940)
4.3% BSA + 0.07% hA1GP53.72 (48.92–58.99)3.481.68 (67.98–98.15)>34.379 (3.567–5.406)3.52.468 (2.042–2.903)2.712.133 (8.236–20.324)2.69.451 (7.111–13.274)2.2
8% BSA + 0.07% hA1GP81.54 (74.25–89.54)5.1101.30 (89.88–114.10)>35.868 (4.831–7.066)4.73.712 (2.598–4.876)4.119.364 (13.335–32.137)4.220.137 (12.445–41.880)4.7
  • a Data represent mean values of three or more independent experiments, and 95% confidence intervals (CI) are in parentheses.

  • b Cytotoxicity was assessed by measurement of the ATP content. Fold change represents the change in the mean 50% cytotoxic concentration (CC50) in the presence of plasma proteins compared to the value in the absence of plasma proteins.

  • c Extracellular HBV DNA was analyzed by extraction of DNA from the cell culture supernatant and quantified by qPCR. Fold change represents the value in the presence of plasma proteins compared to that in the absence of plasma proteins. EC50, 50% effective concentration; EC90, 90% effective concentration.