TABLE 1

Levels of total GSH, TNF-α, and IL-10 in uninfected and M. tuberculosis-infected THP-1 cellsa

Infection status and treatmentGSH concn (μmol/g protein)TNF-α concn (pg/ml)IL-10 concn (pg/ml)
M. tuberculosis uninfected27.64 ± 0.413592.1 ± 104.695.09 ± 10.28
M. tuberculosis infected17 ± 0.017§§§§4,298 ± 392.4§§§75.22 ± 22.58
M. tuberculosis infected plus treatment with:
    NAC32.07 ± 3.663*379.2 ± 91.1###16.52 ± 7.057
    INH26.15 ± 1.114##2,830 ± 787.348.82 ± 19.69
    INH + NAC27.13 ± 2.815#144.9 ± 26.8*, ###12.88 ± 6.316
    RIF20.27 ± 0.040***, ####2,409 ± 312.8##59.12 ± 20.93
    RIF + NAC49.02 ± 7.837*, #170 ± 83.99***, ####29.12 ± 12.08
    EMB47.92 ± 13.732,604 ± 587.8#94.7 ± 29.63
    EMB + NAC70.36 ± 9.504#26.9 ± 1.302**, ####27.48 ± 15.53
    PZA24.02 ± 0.032***, ####2,333 ± 247.2##59.44 ± 26.53
    PZA + NAC29.02 ± 2.536#202.5 ± 50.27****, ###19.76 ± 9.198
  • a The GSH measurements are the mean plus or minus the standard error for the uninfected control group and each infection category. The TNF-α and IL-10 cytokine measurements are the mean plus or minus the standard error for the uninfected control group and each of the infection categories. *, P < 0.05 when the group treated with antibiotic plus NAC is compared to the group treated with antibiotic alone or the M. tuberculosis-infected group treated with NAC is compared to the infected control group; **, P < 0.005 when the group treated with antibiotic plus NAC is compared to the group treated with antibiotic alone or the infected group treated with NAC is compared to the infected control group; ***, P < 0.0005 when the group treated with antibiotic plus NAC is compared to the group treated with antibiotic alone or the infected group treated with NAC is compared to the infected control group; ****, P < 0.00005 when the group treated with antibiotic plus NAC is compared to the group treated with antibiotic alone or the infected group treated with NAC is compared to the infected control group; #, P < 0.05 when the group treated with each antibiotic is compared to the infected control group; ##, P < 0.005 when the group treated with each antibiotic is compared to the infected control group; ###, P < 0.0005 when the group treated with each antibiotic is compared to the infected control group; ####, P < 0.00005 when the group treated with each antibiotic is compared to the infected control group; §§§, P < 0.0005 when the infected control group is compared to the uninfected control group; §§§§, P < 0.00005 when the infected control group is compared to uninfected control group.