TABLE 4

MICs of quinoline-resistant mutants in MG1655 lptD(imp4213)

msbA mutationaMIC (μg/ml)b
G592G913G332CIPPMBTETERM
None10 ± 00.15 ± 0.170.023 ± 00.009 ± 0.0030.23 ± 0.151.2 ± 0.350.18 ± 0.18
A175V>411.9 ± 0.800.20 ± 0.110.009 ± 0.0030.17 ± 0.0581.2 ± 0.350.18 ± 0.18
P176A>413.7 ± 1.60.29 ± 0.100.011 ± 0.0020.23 ± 0.151.0 ± 0.400.20 ± 0.17
P176L>413.7 ± 1.60.29 ± 0.100.011 ± 0.0020.23 ± 0.151.2 ± 0.350.18 ± 0.18
I182M41 ± 02.1 ± 1.20.59 ± 0.590.009 ± 0.0030.23 ± 0.151.2 ± 0.350.18 ± 0.18
M291L34 ± 121.6 ± 1.10.18 ± 0.120.011 ± 0.0020.29 ± 0.171.2 ± 0.350.18 ± 0.18
  • a The amino acid change encoded by mutation of msbA is given.

  • b For all samples, n ≥ 3. CIP, ciprofloxacin; PMB, polymyxin B; TET, tetracycline; ERM, erythromycin.