TABLE 2

Association and dissociation rate constants and apparent binding constants of MEDI4893 for AT alanine mutantsa

Strainkon (M−1 s−1)koff (s−1)KD (nM)Fold loss
WT1.00e+061.40e−040.14
D183A1.78e+069.08e−040.5113.5
S186A7.90e+051.04e−040.1320
N188A2.40e+065.77e−032.40516
P189A2.13e+063.60e−031.69411
V190A2.34e+066.77e−040.2892
R200A1.17e+063.14e−032.6918
T263A1.37e+062.04e−040.150
K266A1.35e+065.15e−033.81226
  • a Association (kon) and dissociation (koff) rate constants were measured using a BIAcore instrument, and the apparent binding constant (KD) was calculated as kon/koff. Data are representative of one of three separate experiments. No binding was observed for the W187A mutant.