TABLE 1

Effect of neuraminidase mutation on IC50 and enzyme kinetics

VirusMean ± SD (fold difference)a
IC50 (nM) for:Km (μM)
ZanamivirOseltamivirLaninamivirPeramivir
WT-H2732.2 ± 1.336.1 ± 5.42.6 ± 2.01.0 ± 0.0511.5 ± 1.5
Mut-Y2731.4 ± 0.5 (1)96.7 ± 10.8 (3)1.63 ± 0.4 (1)80.9 ± 6.8 (85)b12.3 ± 0.8 (1)
WT-D1971.6 ± 0.235.1 ± 3.32.8 ± 1.31.0 ± 0.113.8 ± 1.3
Mut-N1974.5 ± 0.7 (3)66.2 ± 7.5 (2)5.0 ± 1.2 (2)3.0 ± 0.7 (3)30.3 ± 3.3 (3)
  • a Fold difference is from corresponding wild-type values. The data are from three independent experiments.

  • b MUT-Y273 has a significantly higher IC50 for peramivir than that of WT-H273 (Mann-Whitney's U test, P < 0.05).