TABLE 3

Primers used in this studya

Forward primersReverse primers
NameSequence (5′–3′)NameSequence (5′–3′)
hp1174SFCGGGATCCTATTATTGGGGAGGCGCGAThp1174SRCCATCGATAGCTCTGTCCTCATCAAATACTG
hp1174XFAACTGCAGACTTCTAACACTCTGGCGCThp1174XRCGGAATTCCACCGCCAAGCCCAAATAAG
hp1174CFCGGAATTCATGCAAAAAACTTCTAACAChp1174CRCGGGATCCTTAGGAGTTTTCTTCTTGCT
hp0313CGCATGCTTTTTACCCATTThp0313AGAAAACACCACCCACGAAG
hp0936ACGCGCCAAGTTTAGTCAAThp0936CAGGCGCTGCTATAGCTTTT
hp1091TGCGTTCTTAGCGCCTTATThp1091GCGCCATAAGGATAATGGAA
hp1165AGGGAGTTCTTTGGGATCGThp1165AAGACGGGCGTAATCAAATG
hp1174CCGCTGGTAATCCCTTTGTAhp1174CTTGCATTATCGCCCATTTT
hp1181GGGGTGGCGTTTTTCTTTAThp1181CCCCAAATAAGCCCTAAAGC
hp1185ATCTCTGGGCATTTCACCAChp1185CCATCGCAAAAGCGATAAAT
hp1220CAAAAGGCATGAGGGAAAAAhp1220TTGCGTTTTGGCTAAATTCC
hp1082TGCCGTTAGCTGCTATTCCThp1082ACGGCGATGTTTTTGATACC
hp1486AAATGAAGCCCACACCACTChp1486TAAATTCCGCATGCATTTGA
hp1206TTTTCCTGCTTGTGCTGATGhp1206CCCCACCAAGCAAAAACTAA
hp0714GGGTTTTCCCCATTAAGCAThp0714AGAGGCGATGTTGAGCAGTT
  • a Underlining indicates nucleotides that were added at the 5′ end to create a restriction site.