TABLE 2

qRT-PCR analysis of efflux pump expression difference between biofilm-forming and planktonic H. pylori cells

Efflux pump familyLocus tagPredicted functionFold change in expression between biofilm and planktonic cellsP value for significancea
Major facilitator superfamily (MFS)Hp0313Nitrite0.46 ± 0.14*
Hp0936Proline/betaine1.08 ± 0.17
Hp1091Alpha-ketoglutarate0.58 ± 0,03**
Hp1165Multidrug efflux2.66 ± 0.86
Hp1174Glucose/galactose transporter5.75 ± 0.68b***
Hp1181Multidrug efflux3.34 ± 0.31***
Hp1185Sugar efflux0.45 ± 0.27*
ATP-binding cassette (ABC) superfamilyHp1220Multidrug efflux2.99 ± 0.32**
Hp1082Multidrug efflux2.02 ± 0.25
Hp1486Multidrug efflux1.11 ± 0.29
Hp1206Multidrug efflux1.93 ± 0.17**
  • a Significance was determined using the paired Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

  • b The boldface data indicate that the expression levels of the two genes increased greater than 3-fold in biofilm-forming cells compared to those in planktonic cells.