TABLE 6

Strains and plasmids

Strain or plasmidDescriptionaSource or reference
Strains
    MG1655E. coli K-12 F lambda ilvG negative, rfb-50 rph-1ATCC 700926
    CFT073Uropathogenic E. coli isolated from a woman with acute pyelonephritis, Baltimore, MDATCC 700928
    MG1655 msbA-cKOE. coli K-12 with arabinose-inducible conditional knockout of MsbAThis study
    CFT073 msbA-cKOUropathogenic E. coli with arabinose-inducible conditional knockout of MsbA14
    MG1655 lptD(imp4213)E. coli K-12 with permeable outer membraneThis study
    CFT073 lptD(imp4213)Uropathogenic E. coli with permeable outer membrane14
    MG1655 msbA-cKO lptD(imp4213)E. coli K-12 with arabinose-inducible conditional knockout of MsbA and permeable outer membraneThis study
    CFT073 msbA-cKO lptD(imp4213)Uropathogenic E. coli with arabinose-inducible conditional knockout of MsbA and permeable outer membrane14
    K. pneumoniae 700721MGH78578, isolated from sputum from a 66 year-old man, 1994ATCC 700721
    E. cloacae 13047Strain isolated from spinal fluidATCC 13047
    MG1655 msbA-cKO ΔwcaJE. coli with transduced wcaJ deletion from JW2032 into MG1655 msbA-cKOThis study
    MG1655 ΔwcaJE. coliwith transduced wcaJ deletion from JW2032 into MG1655This study
    E. coli JW2032wcaJ::kanR deletion from Keio collection58
    MG1655 ΔtolCE. coli with transduced tolC deletion from JW5503 into MG165514
    E. coli JW5503tolC::kanR deletion from Keio collection58
    CDC 0006MDR E. coli; KPC+, NDMCDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0055MDR E. coli; KPC, NDMCDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0061MDR E. coli; NDMCDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0001MDR E. coli; KPC-3, TEM-1CDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0079MDR E. cloacae; NDMCDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0106MDR E. cloacae; KPC-3, TEM-1CDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0053MDR K. pneumoniae; CTX-M14, DHA-1 OmpF/OmpK35CDC and FDA Antibiotic Resistance Isolate Bank
    CDC 0038MDR K. pneumoniae; NDMCDC and FDA Antibiotic Resistance Isolate Bank
Plasmids
    pBAD24Arabinose-inducible expression vectorATCC 87399
    pBAD24-MsbAPlasmid for arabinose-inducible MsbA overexpressionThis study
    pLDR9Lambda att site integration vectorATCC 77358
    pLDR8Lambda integrase expression vectorATCC 77357
    pCDF-1bIPTG-inducible expression vector with streptomycin resistance markerNovagen
    pKD46Temperature-sensitive plasmid for Red recombinase expressionColi Genetic Stock Center 7669
    pKD4Template plasmid with kanamycin marker flanked by FRT sitesColi Genetic Stock Center 7632
    pKD46-SmpKD46 with streptomycin resistance marker replacing ampicillin resistance markerThis study
    pET28bIPTG-inducible expression vectorNovagen
    pLMG18Low-copy-number IPTG-inducible expression vector with chloramphenicol resistance marker57
    pLMG18-EcMsbALow-copy-number IPTG-inducible expression vector for E. coli MsbAThis study
    pLMG18-AbMsbALow-copy IPTG-inducible expression vector for A. baumannii MsbAThis study
  • a KPC+, strain contains a Klebsiella pneumoniae carbapenemase (KPC); KPC, strain does not contain a KPC; NDM, strain does not contain a New Delhi metallo-β-lactamase (NDM).