TABLE 1

Results of RESIST-4 O.K.N.V. assay performed on positive blood cultures seeded with carbapenemase and non-carbapenemase-producing Enterobacteriaceae

Carbapenemase
type (n)
SpeciesBeta-lactamase gene(s)No. of
isolates
No. of positive RESIST-4 O.K.N.V. assay result on:Sensitivity (%)Specificity (%)
Positive
vials
4-h
subculture
Positive
vials
4-h
subculture
Positive
vials
4-h
subculture
OXA-48 and
variants (30)
E. coliOXA-48101010100100100100
OXA-181333
Klebsiella pneumoniaeOXA-48101010
OXA-244111
Enterobacter cloacaeOXA-48222
Klebsiella oxytocaOXA-48111
OXA-181111
Citrobacter freundiiOXA-48111
Citrobacter koseriOXA-181111
KPC (17)K. pneumoniaeKPC-2131313100100100100
E. cloacaeKPC-2111
KPC-3111
Enterobacter aerogenesKPC-2111
E. coliKPC-2111
NDM (25)K. pneumoniaeNDM-172728.0100100100
NDM-5111
E. coliNDM-1525
NDM-5222
Proteus mirabilisNDM-1303
Morganella morganiiNDM-1202
C. farmeriNDM-1202
E. cloacaeNDM-1101
P. vulgarisNDM-1101
C. freundiiNDM-1101
VIM (8)E. coliVIM-133375.0100100100
K. pneumoniaeVIM-1111
VIM-4101
P. mirabilisVIM-1111
Citrobacter farmeriVIM-1101
Providencia stuartiiVIM-1111
Non-carbapenemase
producing (18)
K. pneumoniaeCTX-M-1 + TEM-1 + OXA-1 + SHV100100100
ESBL100
CTX-M100
ESBL100
E. cloacaeCTX-M-1 + derepressed AmpC + TEM-1 + OXA-1100
CTX-M and derepressed AmpC100
Derepressed AmpC100
E. coliSHV-2a100
CTX-M-15 + TEM-1100
SHV-12100
TEM-52100
CTX-M-15 + OXA-1100
CTX-M-2 + TEM-1100
CTX-M-14100
CTX-M-3100
CTX-M-1 + TEM-1 + OXA-1100
TEM-1 + SHV-1 + OXA-1100
CTX-M-1100