Analog used in combination with NVR 3-778 | Synergy (μM ^{2}%) | Antagonism (μM ^{2}%) | MacSynergy-predicted effect ^{a} | CI value^{b} | Overall CI^{c} | CalcuSynb-predicted effect^{d} | ||
---|---|---|---|---|---|---|---|---|

ED_{50} | ED_{75} | ED_{90} | ||||||

LMV | 5.1 | −14.3 | Additive | 1.0 | 0.8 | 0.8 | 0.9 ± 0.1 | Additive |

TFV | 18.5 | −7.3 | Additive | 0.8 | 0.8 | 0.8 | 0.8 ± 0.06 | Slight to moderate synergy |

ETV | 1.0 | −16.5 | Additive | 1.0 | 0.5 | 0.5 | 0.7 ± 0.4 | Slight to moderate synergy |

↵a Synergy/antagonism volumes at 95% confidence of <25 µM

^{2}% were defined as insignificant, those between 25 and 50 µM^{2}% were defined as minor, those between 50 and 100 µM^{2}% were defined as moderate, and those of >100 µM^{2}% were defined as strong.↵b CI, confidence interval. The confidence intervals at ED

_{50}, ED_{75}, and ED_{90}represents mean values determined from at least 5 different combination ratios.↵c Mean and standard deviation of all confidence interval (CI) values.

↵d The combination effect prediction based on overall confidence interval values is as follows: <0.7 is synergy, 0.7 to 0.9 is slight to moderate synergy, 0.9 to 1.1 is additive, 1.1 to 1.5 is slight to moderate antagonism, and >1.5 is antagonism.