TABLE 4

Synergy/antagonism analysis for NVR 3-778 in combination with nucleoside analogs using MacSynergy and CalcuSyn

Analog used in combination
with NVR 3-778
Synergy
(μM2%)
Antagonism
(μM2%)
MacSynergy-predicted
effecta
CI valuebOverall CIcCalcuSynb-predicted effectd
ED50ED75ED90
LMV5.1−14.3Additive1.00.80.80.9 ± 0.1Additive
TFV18.5−7.3Additive0.80.80.80.8 ± 0.06Slight to moderate synergy
ETV1.0−16.5Additive1.00.50.50.7 ± 0.4Slight to moderate synergy
  • a Synergy/antagonism volumes at 95% confidence of <25 µM2% were defined as insignificant, those between 25 and 50 µM2% were defined as minor, those between 50 and 100 µM2% were defined as moderate, and those of >100 µM2% were defined as strong.

  • b CI, confidence interval. The confidence intervals at ED50, ED75, and ED90 represents mean values determined from at least 5 different combination ratios.

  • c Mean and standard deviation of all confidence interval (CI) values.

  • d The combination effect prediction based on overall confidence interval values is as follows: <0.7 is synergy, 0.7 to 0.9 is slight to moderate synergy, 0.9 to 1.1 is additive, 1.1 to 1.5 is slight to moderate antagonism, and >1.5 is antagonism.