TABLE 1

Liquid MIC values of strains overexpressing wild-type and mutant ScLDM6×His

StrainGenotypeMIC80 (nM)a
FLCVCZVT-1161ITCPCZ
Y2411AD2Δ pABC31,800 ± 5037 ± 0.929 ± 891 ± 4160 ± 48
Y2300AD2Δ PDR5::ScLDM6×His4,200 ± 50093 ± 1075 ± 7180 ± 6423 ± 60
Y2301AD2Δ PDR5::ScLDM6×His Y140F ΔERG118,100 ± 500202 ± 50172 ± 29146 ± 8366 ± 119
Y2513AD2Δ PDR5::ScLDM6×His Y140H ΔERG118,800 ± 1100164 ± 9116 ± 24165 ± 4330 ± 34
  • a MIC80 determinations were carried out as described in Materials and Methods using SD medium at pH 6.8. Each determination was carried out at least 3 times using samples in triplicate.