TABLE 4

Comparison of 4 plate types in Mueller-Hinton broth versus MRSA ATCC 43300 in the presence and absence of Tween 80 (polysorbate 80) or lysed horse blood (LHB), with added 50% human serum (HS) or 2% lung surfactant (LS)

PlateMediumAdditiveMIC (µg/ml) by antibiotic
VancomycinCiprofloxacinTelavancinDalbavancinMCC223MCC310MCC520
Corning NBSMHNone0.50.125 to 0.250.03 to 0.060.03 to 0.06≤0.003≤0.0030.007
50% HS0.25 to 0.510.25>80.1250.1250.03
2% LS0.50.125 to 10.1250.25 to 0.50.03 to 0.060.015 to 0.030.015 to 0.03
MH + T80None10.50.1250.125 to 0.50.003 to 0.0070.0070.015
50% HS10.125 to 0.250.25>80.1250.060.03 to 0.06
2% LS0.25 to 0.50.25 to 0.50.06 to 0.1250.25 to 0.50.1250.06 to 0.1250.03
MH + 2% LHBNone10.06 to 0.50.06 to 0.1250.06 to 0.250.06 to 0.1250.060.03
50% HS0.5 to 10.25 to 0.50.2580.1250.06 to 0.1250.06 to 0.125
2% LS0.5 to 10.5 to 10.1250.250.250.015 to 0.060.015
Corning PSMHNone0.50.250.060.5 to 10.5 to 140.125 to 0.25
50% HS0.25 to 0.50.25 to 10.125 to 0.25>80.06 to 0.250.06 to 0.1250.06 to 0.25
2% LS0.5 to 10.5 to 20.03 to 0.1251 to 21 to 21 to 20.03 to 0.06
MH + T80None1 to 20.25 to 0.50.03 to 0.060.03 to 0.1250.003 to 0.007≤0.0030.007
50% HS0.50.1250.25>80.125 to 0.250.06 to 0.1250.03 to 0.125
2% LS0.5 to 20.25 to 0.50.06 to 0.250.25 to 0.50.125 to 0.250.06 to 0.250.03 to 0.06
MH + 2% LHBNone10.25 to 10.060.25 to 0.50.25 to 0.50.25 to 0.5≤0.003
50% HS0.5 to 10.03 to 0.50.125 to 0.25>80.25 to 0.50.125 to 0.250.03 to 0.06
2% LS0.5 to 10.50.1250.125 to 0.250.25 to 0.50.25 to 10.015 to 0.03
Corning TCMHNone0.50.06 to 0.125141 to 22 to 42 to 4
50% HS0.25 to 0.50.5 to 10.5 to 1>84 to >84 to >81 to >8
2% LS0.25 to 10.125 to 0.50.25 to 0.514 to 82 to 41
MH + T80None10.125 to 0.50.125 to 0.250.125 to 0.251 to 22 to 44
50% HS0.5 to 10.25 to 10.25 to 1>82 to 84 to 82
2% LS0.50.5 to 10.125 to 0.250.25 to 0.544 to 82
MH + 2% LHBNone0.50.1250.1250.06 to 0.52 to 42 to 81 to 4
50% HS0.50.25 to 12 to 882 to 80.5 to 42 to 4
2% LS0.5 to 10.50.25 to 10.25 to 0.54 to >842 to 4
Trek PSMHNone0.5 to 10.5 to 10.03 to 0.060.25 to 0.50.5 to 140.125
50% HS0.250.25 to 20.125>80.06 to 0.250.06 to 0.1250.003 to 0.015
2% LS0.030.125 to 0.250.03 to 0.0610.25 to 0.50.25 to 10.25 to 1
MH + T80None10.25 to 0.50.03 to 0.060.06≤0.003≤0.0030.007
50% HS0.25 to 0.50.25 to 0.50.125 to 0.258 to >80.1250.060.03 to 0.06
2% LS0.06 to 10.25 to 0.50.060.250.250.250.015
MH + 2% LHBNone0.250.06 to 0.1250.0150.1250.1250.1250.003 to 0.015
50% HS0.50.25 to 0.50.06 to 0.1258 to >80.125 to 0.250.06 to 0.50.06 to 0.25
2% LS0.25 to 0.50.1250.06 to 0.250.1250.250.125≤0.003