TABLE 5

Comparison of 4 plate types in Mueller-Hinton broth versus E. coli ATCC 25922 in the presence and absence of Tween 80 (polysorbate 80) or lysed horse blood (LHB), with added 50% human serum (HS) or 2% lung surfactant (LS)

PlateMediumAdditiveMIC (µg/ml) by antibiotic
ColistinCiprofloxacinOxacillinTrimethoprimPolymyxin BPenicillin GRifampin
Corning NBSMHNone≤0.03≤0.03>640.25 to 0.5≤0.03644 to 8
50% HS≤0.03≤0.03>641 to 2≤0.03648
2% LS≤0.03≤0.03>641 to 2≤0.03648
MH + T80None≤0.03≤0.03>641≤0.03648
50% HS≤0.03≤0.03>6420.03 to 0.0632 to 648
2% LS0.03 to 0.06≤0.03>642≤0.03324 to 8
MH + 2% LHBNone≤0.03≤0.03>641≤0.0332 to 642 to 4
50% HS≤0.03≤0.03>641 to 2≤0.0332 to 648
2% LS0.03 to 0.060.03 to 0.06>641≤0.0332 to 644 to 8
Corning PSMHNone0.06≤0.03>640.25 to 0.50.0632 to 644 to 8
50% HS≤0.03≤0.03>641 to 2≤0.03644 to 8
2% LS0.06 to 0.25≤0.03>6410.06 to 0.12564 to >648
MH + T80None≤0.03≤0.03>641 to 20.03 to 0.25648 to 16
50% HS≤0.03≤0.03>641 to 20.03 to 0.06648
2% LS0.03 to 0.06≤0.03>641 to 20.06 to 0.12532 to 644 to 8
MH + 2% LHBNone1≤0.03>641 to 22 to 8644 to 8
50% HS≤0.03≤0.03>641 to 80.03 to 0.0632 to >644 to 8
2% LS0.06 to 0.125≤0.03>641 to 20.0632 to 644 to 8
Corning TCMHNone0.5 to 1≤0.03>640.25 to 0.51 to 2324 to 8
50% HS0.125 to 0.5≤0.03>640.5 to 10.25 to 0.5648
2% LS0.5 to 1≤0.03>641 to 24648
MH + T80None1≤0.03>640.5 to 12648
50% HS0.25 to 0.5≤0.03>641 to 21 to 4648
2% LS1 to 2≤0.03>641 to 22 to 432 to 644 to 8
MH + 2% LHBNone≤0.03≤0.03>6410.125648
50% HS0.25 to 0.5≤0.03>641 to 2432 to 648 to 16
2% LS2 to 4≤0.03>641 to 22 to 832 to 648
Trek PSMHNone≤0.03≤0.03>640.5 to 10.06 to 0.125644
50% HS≤0.03≤0.03>641≤0.0364 to >648
2% LS≤0.03≤0.03>641 to 40.5 to 1644 to 8
MH + T80None≤0.03≤0.03>6420.03 to 0.0632 to 648 to 16
50% HS≤0.03≤0.03>642 to 40.03 to 0.125324 to 8
2% LS≤0.03≤0.03>6420.03 to 0.06324
MH + 2% LHBNone0.03 to 0.060.03 to 0.06>6410.03 to 0.25324 to 8
50% HS0.03 to 0.06≤0.03>641≤0.03324
2% LS≤0.03≤0.03>640.5 to 1≤0.03322 to 4