TABLE 2

MICs of daptomycin, AFN-1252, and oxacillin in relevant growth media

Antibiotic and mediumaMIC (μg ml−1)
USA300 WTUSA300 ΔagrACC6CC7CC9CD1CD2CD3CD4CD5CD6CD8
Daptomycin
    TSB0.5Ca111112211111
    TSB1.25Ca0.50.50.250.250.250.50.50.250.250.50.250.25
    MHB1.25Ca0.250.250.250.250.250.250. 50.250.250.250.250.25
    TSB/serum0.50.50.50.50.5110.50.50.50.50.5
AFN-1252
    TSB0.5Ca0.0150.0150.0150.0080.0150.0150.0150.0150.0150.0150.0150.008
    TSB1.25Ca0.0150.0150.0150.0080.030.0150.030.0150.030.0150.0150.008
    MHB1.25Ca0.0150.0150.0150.0080.0150.0150.0150.0080.0150.0080.0080.008
    TSB/serum0.060.060.060.060.060.060.060.060.060.060.060.06
Oxacillin
    TSB0.5Ca443284>1283212832161616
    TSB1.25Ca44884>128812816888
    MHB1.25Ca22822>12841288422
    TSB/serum8864168>1283212832323232
  • a TSB0.5Ca, TSB containing 0.5 mM CaCl2; TSB1.25Ca, TSB containing 1.25 mM CaCl2; MHB1.25Ca, Mueller-Hinton broth containing 1.25 mM CaCl2; TSB/serum, TSB containing 1.25 mM CaCl2 and 50% normal human serum.