TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    B. licheniformis DC-1Polymyxin-degrading bacteriumLaboratory stock
    B. subtilis WB800Host for expression24
    B. subtilis WB800/pWBUC01B. subtilis WB800 with pWBUC01This study
    B. subtilis WB800/pWBUC01-aprB. subtilis WB800 with pWBUC01-aprThis study
    E. coli DH5αColistin sensitive, host for cloningLaboratory stock
    Micrococcus luteusGramicidin and daptomyxin sensitiveLaboratory stock
Plasmids
    pWBUC01Apr Kmr, E. coli-B. subtilis shuttle vectora27
    pWBUC01-aprpWBUC01 carrying apr and its 5′ UTRThis study
  • a Apr, Ampicillin resistance; Kmr, kanamycin resistance.