TABLE 2

Activity of APX001A and comparator agents tested against Candida spp. and Cryptococcus neoformans using the CLSI broth microdilution method

Organism and antifungal agentMIC (μg/ml)CLSI category (%)aECV category (%)b
MIC50MIC90RangeSI/SDDRWTNWT
C. albicans (414 isolates tested)
    APX001A0.0080.008≤0.002–0.03100.00.0
    Anidulafungin0.0150.03≤0.008–199.80.00.299.80.2
    Micafungin0.0150.015≤0.008–299.80.00.299.30.7
    Fluconazole≤0.120.25≤0.12–899.50.20.298.81.2
    Posaconazole0.030.06≤0.008–0.2593.26.8
    Voriconazole≤0.0080.015≤0.008–0.12100.00.00.099.80.2
    Amphotericin B0.510.25–1100.00.0
C. glabrata (321 isolates tested)
    APX001A0.060.120.004–0.25100.00.0
    Anidulafungin0.060.120.015–494.42.23.496.63.4
    Micafungin0.0150.03≤0.008–296.90.32.895.05.0
    Fluconazole432≤0.12–>12891.68.488.511.5
    Posaconazole0.510.03 –>492.87.2
    Voriconazole0.061≤0.008–>488.811.2
    Amphotericin B110.25–2100.00.0
C. parapsilosis (270 isolates tested)
    APX001A0.0080.015≤0.002–0.0698.91.1
    Anidulafungin220.015–493.76.30.0100.00.0
    Micafungin110.015–2100.00.00.0100.00.0
    Fluconazole0.252≤0.12–3290.72.27.088.911.1
    Posaconazole0.060.120.015–0.599.60.4
    Voriconazole≤0.0080.06≤0.008–0.593.36.70.089.310.7
    Amphotericin B0.510.5–1100.00.0
C. tropicalis (151 isolates tested)
    APX001A0.0150.030.004–0.06100.00.0
    Anidulafungin0.030.06≤0.008–0.25100.00.00.098.71.3
    Micafungin0.030.06≤0.008–0.12100.00.00.097.42.6
    Fluconazole0.250.5≤0.12–3298.00.02.096.73.3
    Posaconazole0.060.120.015–0.2590.79.3
    Voriconazole0.0150.06≤0.008–198.01.30.798.02.0
    Amphotericin B0.510.5–1100.00.0
C. krusei (43 isolates tested)
    APX001A>2>2>2–>2
    Anidulafungin0.060.120.015–0.12100.00.00.0100.00.0
    Micafungin0.060.12≤0.008–0.12100.00.00.0100.00.0
    Fluconazole323216–64
    Posaconazole0.50.50.06–0.5100.00.0
    Voriconazole0.250.50.12–197.72.30.097.72.3
    Amphotericin B111–2100.00.0
C. dubliniensis (49 isolates tested)
    APX001A0.0040.008≤0.002–0.015100.00.0
    Anidulafungin0.030.120.015–0.12100.00.0
    Micafungin0.0150.03≤0.008–0.03100.00.0
    Fluconazole≤0.120.25≤0.12–298.02.0
    Posaconazole0.030.060.015–0.12
    Voriconazole≤0.0080.015≤0.008–0.03
    Amphotericin B0.250.5≤0.12–0.5
C. lusitaniae (39 isolates tested)
    APX001A0.030.120.015–0.5
    Anidulafungin0.250.50.12–0.5100.00.0
    Micafungin0.120.250.06–0.5100.00.0
    Fluconazole0.252≤0.12–6489.710.3
    Posaconazole0.060.120.03–0.2564.135.9
    Voriconazole≤0.0080.015≤0.008–0.5
    Amphotericin B0.510.25–1
C. kefyr (13 isolates tested)
    APX001A0.120.50.06–0.5
    Anidulafungin0.060.120.03–0.12
    Micafungin0.030.060.03–0.06
    Fluconazole0.250.25≤0.12–0.5
    Posaconazole0.120.120.06–0.25
    Voriconazole≤0.008≤0.008≤0.008–0.015
    Amphotericin B110.5–1
Cryptococcus neoformans var.
grubii (30 isolates tested)
    APX001A0.250.50.03–2
    Anidulafungin>4>42–>4
    Micafungin>4>44–>4
    Fluconazole240.5–4100.00.0
    Posaconazole0.120.250.03–0.25100.00.0
    Voriconazole0.030.060.015–0.12100.00.0
    Amphotericin B0.510.25–161.538.5
  • a CLSI breakpoint criteria for susceptible (S), intermediate/susceptible dose-dependent (I/SDD), and resistant (R).

  • b The WT-UL MIC value was used in place of the ECV to designate WT and NWT strains for APX001A.