TABLE 4

rdotp values and R ratios for 24 blaNDM-containing isolatesa

Sample no.CDC/FDA AR
bank or NIH
isolate
SLGNLGDADTEHYAASAR
rdotp/R value
SLGNLGDADTEHYAASAR
single-peptide call
ASMIVMSHSAPDSR
rdotp/R value
ASMIVMSHSAPDSR
single-peptide call
AFGAAFPK
rdotp/R value
AFGAAFPK
single-peptide call
Final callConcn
(fm/μg)
L002CDC_1481/6.87Positive0.99/6.21Positive1/3.05PositivePositive85.9
L004CDC_1501.0/17.0Positive0.98/18.7Positive1.0/7.36PositivePositive212.5
L008CDC_0571.0/0.58Positive0.99/0.55Positive0.98/0.35PositivecPositive7.3
L016CDC_1521.0/8.02Positive0.98/6.72Positive1.0/3.06PositivePositive100.3
L024CDC_0381.0/9.46Positive0.98/8.82Positive1.0/3.83PositivePositive118.3
L027CDC_1431.0/5.25Positive0.99/3.47Positive0.99/2.01PositivePositive65.6
L030CDC_1461.0/9.71Positive0.98/6.44Positive1.0/3.55PositivePositive121.4
L032CDC_1511.0/51.02Positive0.99/38.15Positive1.0/19.44PositivePositive637.8
L048NDM-331.0/40.3Positive0.98/31.52Positive1.0/15.83PositivePositive503.8
L050CDC_1581.0/5.58Positive0.98/3.41Positive1.0/1.94PositivePositive69.8
L051CDC_1271.0/17.48Positive0.99/16.15Positive1.0/6.5PositivePositive218.5
L054CDC_1621.0/48.27Positive0.99/48.01Positive1.0/19.33PositivePositive603.4
L057CDC_1491.0/39.65Positive0.97/40.2Positive1.0/15.37PositivePositive495.6
L063CDC_1370.95/0.01Negativeb0.98/28.08Positive1.0/12.28PositivePositivee351.0
L075CDC_0821.0/0.28Positived0.94/0.33Positivec,d,e0.93/0.2Positivec,dPositive3.5
L082CDC_1451.0/5.54Positive0.98/4.21Positive0.99/2.05PositivePositive69.3
L086CDC_1391.0/5.08Positive0.99/4.18Positive1.0/2.04PositivePositive63.5
L089CDC_1571.0/12.3Positive0.96/9.39Positive1.0/4.94PositivePositive153.8
L094CDC_1191.0/1.87Positive0.98/1.68Positive1.0/0.81positvePositive23.4
L098CDC_1381.0/86.58Positive0.98/64.12Positive1.0/30.76PositivePositive1,082.3
L100CDC_0371.0/0.11Positived0.93/0.08positivec,d,e0.94/0.11Possible positivePositive1.4
L017NDM-340.99/0.05Negative0.96/0.04Negative0.95/0.23cNegativeNegativeNot detectable
L017gNDM-340.94/0.04Negative0.94/0.04Negative0.93/0.10cNegativeNegativehNot detectable
L092CDC_1180.94/0.02Negativeb0.79/0.03negative0.85/0.05NegativecNegativeNot detectable
L092gCDC_1181.0/17.9Positive1.0/32.5Positive1.0/7.8PositivePositiveh223.8
L099CDC_1280.99/0.05Negativef0.99/0.05Negativec0.94/0.04Negativeb,cNegativeNot detectable
L099gCDC_1281.0/26.2Positive1.0/50.6Positive1.0/11.2PositivePositiveh327.5
  • a Three repeated tests for L017, L092, and L099 were also included in the analysis; those data were not included in the calculations of sensitivity and specificity.

  • b Noise.

  • c Interference.

  • d Weak signal.

  • e Removal of one transition.

  • f Carryover.

  • g A new preparation was tested after 100-sample validation was completed. The values reported represent averages of results from three replicates.

  • h These data were not included in the calculation of assay sensitivity and specificity.