TABLE 1

The types of mutations in evolved populations of WT PAO1 and P. aeruginosa ΔkatA

Strain and conditionNo. (%) of mutations by typeaTotal no. of mutationsdN/dS
IndelTransitionTransversionLarge indelInversion duplication
PAO1
    CTRL biofilm24 (28)13 (15)48 (56)850.35
    CTRL planktonic24 (26)22 (23)48 (51)940.25
    CIP biofilm60 (37)27 (16)77 (47)1640.44
    CIP planktonic29 (22)44 (34)57 (44)1300.45
ΔkatA strain
    CTRL biofilm147 (22)58 (9)452 (69)16581.36
    CTRL planktonic141 (24)70 (12)362 (63)55781.24
    CIP biofilm301 (28)206 (19)557 (52)2910750.90
    CIP planktonic150 (22)65 (10)469 (69)676972.59
  • a The numbers and types of mutations in evolved PAO1 populations were reanalyzed including nonsynonymous mutations in hypothetical proteins (not included in reference 23).