TABLE 4

Activity of GC-072 and comparators against efflux-proficient strains of B. pseudomallei

TABLE 4
  • a All strains used in this study are derived from B. pseudomallei 1026b as previously described, with the exception of Bp282 and Bp320. The latter two strains were derived as follows: Bp282 is a ciprofloxacin-resistant derivative of Bp207 obtained using passive selection. It contains a bpeT point mutation causing an S280P BpeT amino acid substitution. The passive selection experiment was conducted prior to 4 December 2012, and its performance and mutant possession did not require US Federal Select Agent Program approval. Bp320 is a Δ(bpeEF-oprC) derivative of Δ(amrAB-oprA) Δ(bpeAB-oprB) strain Bp282 and was constructed using the previously described methods. This strain does not express any known efflux pumps (48).

  • b The MIC against each strain was determined using broth microdilution assays. The data shown represent three biological replicates, each in technical duplicates, reporting modes from 6 data points.

  • c The control strains E. coli ATCC 25922 and P. aeruginosa ATCC 27853 were verified in parallel with all MIC testing to ensure that MICs for the control antibiotics were within the expected range, according to CLSI guidelines.