TABLE 1

IC50 values determined for compounds tested on Naegleria gruberi and N. fowleria

TABLE 1
  • a N. gruberi growth curve data were obtained by measuring optical density daily over a 5-day period, after which area under the growth curve values were calculated. The IC50 data from compounds tested on N. gruberi represent approximations, as the range of concentrations tested was narrow. N. fowleri was tested in two ways: with CellTiter-GLO ATP stain and through cell counting with a guava EasyCyte flow cytometer. Levels of CellTiter-GLO luminescence were determined after 24 h of incubation, and cell counts were determined after 72 h of incubation. Raw data were normalized as a percentage of the levels measured for the respective controls. Nonlinear regression was performed by the use of GraphPad Prism 8 as [inhibitor] versus normalized response with a variable slope, after which the IC50 data were calculated. Acyl-CoA, acyl-coenzyme A; CI, confidence interval.

  • b —, calculation not possible.