TABLE 1.

Potential biomarkers identified from iTRAQa

Time (h)Common name of targetLocus tagSusceptible iTRAQ fold changeFractionResistant iTRAQ fold changeFold change by microarray
24Mitochondrial hypoxia-responsive domain proteinAFUA_1G12250>−16SecretedToo low−4.5
24Class III chitinase ChiA1AFUA_5G03760−12.1CW/PMNI−11.7
24Mitochondrial hypoxia-responsive domain proteinAFUA_1G12250−6.1CW/PM1.15−4.5
24Plasma membrane H+-ATPase Pma1AFUA_3G07640−4.3CW/PM−1.15−5.1
2460S ribosomal protein L7AFUA_4G038802.0CW/PM−1.073.8
2460S ribosomal protein L23AFUA_5G056302.0CW/PMNI4.5
2460S ribosomal protein L22, putativeAFUA_3G123002.0CW/PM1.0710.2
2440S ribosomal protein S6AFUA_4G108002.0CW/PM−1.073.7
2440S ribosomal protein S9AFUA_3G069702.0CW/PM−1.073.8
2460S ribosomal protein P0AFUA_1G050802.1CW/PM−1.234.8
2460S ribosomal protein L11AFUA_4G077302.1CW/PMNI8.3
2460S ribosomal protein L44AFUA_2G081302.1CW/PM1.413.5
2440S ribosomal protein S3AeAFUA_5G054502.3CW/PM1.074.9
24Ribosomal protein S5AFUA_7G014602.3CW/PMNo Change3.2
24Ribosomal L18ae protein familyAFUA_1G045302.3CW/PMNI3.8
2440S ribosomal protein S10bAFUA_6G126602.3CW/PM1.073.8
24Ribosomal protein S13p/S18eAFUA_6G135502.3CW/PM−1.235.0
2460S ribosomal protein L35AeAFUA_3G084602.3CW/PMNI5.3
24Cytosolic large ribosomal subunit protein L7AAFUA_6G129902.5CW/PM1.073.9
48BYS1 domain protein, putativeAFUA_5G01990−2.5CW/PM1.232.7
48Translation elongation factor eEF-3, putativeAFUA_7G056602.3CW/PM1.07−2.7
48GPI-anchored cell wall organization protein Ecm33AFUA_4G068202.3Secreted1.07−2.2
  • a NI, not identified; too low, not enough signal to detect the reporter ion; GPI, glycosylphosphatidylinositol; CW/PM, cell wall/plasma membrane.