Table 1.

Primers used in this study

PrimerSequenceaNucleotide positionb
ampC-F5′-ATG ACG AAA GTG AAC CGC-3′1-18
ampC-R5′-CCT GGA CGA TGT GGT AAG-3′1103-1120
CTX-M-3F5′-ACC GTC ACG CTG TTG TTA G-3′47-64
CTX-M-3R5′-CTT TCT GCC TTA GGT TGA G-3′806-824
IMP-1F5′-ACC GCA GCA GAG TCT TTG CC-3′49-68
IMP-1R5′-ACA ACC AGT TTT GCC TTA CC-3′616-635
IMP-2F5′-GTT TTA TGT GTA TGC TTC C-3′16-34
IMP-2R5′-AGC CTG TTC CCA TGT AC-3′677-693
VIM-2F5′-ATG TTC AAA CTT TTG AGT AAG-3′1-21
VIM-2R5′-CTA CTC AAC GAC TGA GCG-3′784-801
NDM-1F5′-TTG CCC AAT ATT ATG CAC CC-3′7-26
NDM-1R5′-ATT GGC ATA AGT CGC AAT CC-3′407-426
16S rRNA-10F5′-GTT TGA TCC TGG CTC A-3′11-26
16S rRNA-800R5′-TAC CAG GGT ATC TAA TCC-3′785-802
SMB-F5′-CAG CAG CCA TTC ACC ATC TA-3′79-98
SMB-R5′-GAA GAC CAC GTC CTT GCA CT-3′551-570
SMB-CloF5′-CCC AAG CTT TCC GCC GAC TTG GCG CAG-3′
SMB-CloR5′-GGG GTA CCA AGA CCG ATT TAG CCG GC-3′
PET-15′-GGA ATT CCA TAT GAA AAT CAT CGC TTC CC-3′
PET-25′-CCC AAG CTT TCA GCG TTT CTC GCT GGC C-3′
  • a Underlines indicate the sites for restriction endonuclease.

  • b Position numbers correspond to the nucleotides of the coding sequences. Position numbers are assigned to the primers that amplify the internal region of the coding sequences.