Table 1.

Origin, structure of Tn4401, other β-lactamases, plasmid analysis, pulsotype, and sequence type of the P. aeruginosa isolates

IsolateOrigin in ColumbiaYr of isolationSite of isolationLocationPCR and sequencing result (Tn4401 homology)blaKPC-2-positive plasmidBacterial isolate characteristic
NameSize (kb)TransformantPulsotypeSequence type
blaKPC-2TnpAISKPN6ISKPN7ECaPAb
PA-1Medellin2006Bronchial secretionTertiary care centerKPC-2+++pCOL45++A1ST308
PA-2Bogota2006StoolUnknownKPC-2+pPA-213++BST1006
PA-3Barranquiila2006Bronchial secretionIntensive care unitKPC-2+++pPA-360CST1060
PA-4Medellin2006BoneGeneral wardcKPC-2+++pPA-445++A2ST308
PA-5Medellin2006Surgical woundGeneral wardKPC-2+++pPA-545++A2ST308
PA-6Medellin2006Surgical woundGeneral wardKPC-2+++pPA-645++A1ST308
PA-7Medellin2006UrineGeneral wardKPC-2+++pPA-745++A1ST308
PA-8Medellin2006Tracheal aspirateIntensive care unitKPC-2+++pPA-845++A1ST308
PA-9Cali2007Peritoneal fluidGeneral wardKPC-2+++DST235
PA-10Pereira2010UrineIntensive care unitKPC-2+++EST235
  • a Transformants into E. coli (EC) DH10B.

  • b Transformants into P. aeruginosa (PA) KG2505.

  • c General ward: patients hospitalized for surgical or medical disease.