Table 3.

phoPQ alleles and associated Pm susceptibility

Allele designationStrain no.Amino acid change(s)aPm susceptibilityb
PhoPPhoQ
phoPQWTWTS
phoPQ6WTframeshift at I421 (and 8 amino acids)R
phoPQ11WTI421XR
phoPQ12WTD433XR
phoPQ211018 (and 9 other isolates)WTΔL364-G365R
phoPQ221582WTΔL364-G365; R444CR
phoP23Q231565M175IR6C; ΔL364-G365I
phoP24Q241604 (and 2 other isolates)R118CΔV57-Q332S
phoPQ24WTΔV57-Q332R
phoPQ251571WTframeshift at V448 (and 30 amino acids)S
phoPQ301016WTE72GS
  • a C, Cys; D, Asp; E, Glu; G, Gly; I, Ile; L, Leu; M, Met; Q, Gln; R, Arg; V, Val; X, Ter.

  • b Alleles were inserted into pJN105D and expressed in PAK ΔphoPQ, with Pm susceptibility determined by alternative PMB plate assays and interpreted as defined in Materials and Methods. S, susceptible, I, intermediate, and R, resistant.