Table 4.

Expression of genes of smeRv-smeU1-V-W-U2-X regulon for strains KJ, KJ09C and its derived mutant, determined by qRT-PCR

GeneRelative fold change of transcripta
KJKJ09CKJ09CΔRvKJ09CΔU1KJ09CΔVWKJ09CΔU2KJ09CΔX
smeRv178.8 ± 6.10.36 ± 0.082.1 ± 7.7174.4 ± 18.751.6 ± 4.147.17 ± 3.9
smeU1134.3 ± 2.30.95 ± 0.12.1 ± 0.142.2 ± 5.747.5 ± 5.948.5 ± 2.4
smeV122.5 ± 1.43.38 ± 0.410.4 ± 0.61.7 ± 0.121.5 ± 2.912.0 ± 0.5
smeW158.9 ± 4.40.77 ± 0.241.9 ± 3.71.1 ± 0.919.2 ± 1.620.8 ± 1.8
smeU21156.5 ± 13.41.82 ± 0.8178.5 ± 15.4179.7 ± 15.30.2 ± 0.0108.4 ± 14.3
smeX124.9 ± 2.70.31 ± 0.034.7 ± 4.026.7 ± 3.86.4 ± 0.70.62 ± 0.0
  • a Relative fold changes between wild-type KJ and its derived mutant were calculated by the 2ΔΔCT method.