Table 5.

Determination of C23O activities of strain KJ and its derived mutants containing different transcriptional fusion constructs

StrainC23O activity (Uc/OD450)a
KJ(p371RvxylE)5 ± 0.7
KJΔRv(p371RvxylE)58 ± 6.1
KJ09C(p371RvxylE)305 ± 41
KJ09CΔRv(p371RvxylE)21 ± 1.9
KJ(p371U1xylE)1 ± 0.4
KJΔRv(p371U1xylE)3 ± 0.7
KJ09C(p371U1xylE)25 ± 3.1
KJ09CΔRv(p371U1xylE)2 ± 0.5
KJ(p272XxylE)4 ± 0.4
KJ09C(p272XxylE)5 ± 0.5
  • a Uc is defined as 1 nanomole of catechol hydrolyzed per minute. Results are expressed as means ± SDs of three independent determinations. OD450, optical density at 450 nm.