Table 1.

Ribosomal mutations and erythromycin susceptibility in Campylobacter jejuni strains compared to C. jejuni DSM 4688 type straine

Strain(s)Erythromycin MIC (μg/ml)d23S rRNA gene mutationaL4 mutation(s)L22 mutation(s)L22 insertion
DSM 4688b0.5
1>128wtA121V, I192MA74G, A109T, E111A, T114A, A130T, A132V, A141V, K144E73-GAVLT-74
2–6>128A2059GA121V, I192MA74G, A109T, E111A, T114A, A130T, A132V, A141V, K144E
7>128A2059GwtA74G, A103V, A141V, K144E
8c>128A2059GwtA74G, A103V, A141V
9, 10>128A2059GA121V, I192M, A196VV65I, A109S, A143V
11>128A2059GA121V, I192MQ24R, V65I, A143V
12>128A2059GA121V, I192MV65I, A103V
13>128A2059GA121V, I192MG86E
14128A2059GA121V, I192MA74G, A109T, E111A, T114A, A130T, A132V, A141V, K144E
15128A2059GwtV65I, A103V
1664wtT177SI65V, D72N
17, 188wtA121V, I192MQ24R, V65I, A143V
198wtA121V, I192MV65I, A109S, A143V
20, 224–8wtA121V, I192MV65I, A103V
214wtN95S, A121V, I192MQ24R, V65I, A143V
232wrA129T, T201Iwt
24, 251wtA121V, I192MV65I, A109S, A143V
26, 30, 310.5–1wtA121V, I192M, A196VV65I, A109S, A143V
271wtwtV65I, A103V
28, 291wtT177SV65I
320.5wtV80I, A121V, I192MV65I, A103V
330.5wtS132N, T177SV65I
340.5wtA140Twt
  • a Position according to Escherichia coli numbering.

  • b Reference strain for the ribosomal proteins L4 and L22.

  • c Only two mutated copies of A2059G.

  • d From each MIC, a number of strains were selected for the efflux pump inhibitor (NMP and PAβN) testing, and no decrease in MIC was observed.

  • e Abbreviations: A, Ala; D, Asp; E, Glu; G, Gly; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; wt, wild type, based on corresponding sequences of C. jejuni DSM 4688 type strain.