TABLE 1.

Yeast strains and plasmids used in this studya

Strain, genotype or plasmidDescriptionSource and/or reference
Strains and genotypes
    S288CMATα SUC2 mal mel gal2 CUP1 flo1 flo8-1ATCC
    W303-1AMATa ura3-1 leu2-3,112 trp1-1 can1-100 ade2-1 his3-11,15 [psi+]Joseph Heitman (DU)
    BY4743MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lys2Δ0Open Biosystems, AL
    BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0Open Biosystems, AL
    pmr1Δpmr1Δ in BY4742Open Biosystems, AL
    pmr1/pmr1pmr1Δ/Δ in BY4743Open Biosystems, AL
    pmc1/pmc1pmc1Δ/Δ in BY4743Open Biosystems, AL
    cch1/cch1cch1Δ/Δ in BY4743Open Biosystems, AL
    mid1/mid1mid1Δ/Δ in BY4743Open Biosystems, AL
    cna1/cna1cna1Δ/Δ in BY4743Open Biosystems, AL
    cna2/cna2cna2Δ/Δ in BY4743Open Biosystems, AL
    cnb1/cnb1cnb1Δ/Δ in BY4743Open Biosystems, AL
    crz1/crz1crz1Δ/Δ in BY4743Open Biosystems, AL
Plasmids
    YCplac33CEN backbone plasmid with heat shock promoter elements20, Rajini Rao (JHU)
    YCpHR4Wild-type PMR1 in YCplac3320, Rajini Rao (JHU)
    Q783AContains mutant PMR1 (defective in Mn2+ transport)20, Rajini Rao (JHU)
    D53AContains mutant PMR1 (defective in Ca2+ transport)20, Rajini Rao (JHU)
    pAMS3664X-CDRE::lacZ reporter32, Kyle Cunningham (JHU)
  • a DU, Duke University; JHU, Johns Hopkins University; CDRE, calcineurin-dependent response element; CEN, centromere.