Table 3.

Properties of NISr mutants selected from S. aureus SH1000

StrainNIS MIC (μg/ml)Doubling time (min) (mean ± SD)Relative competitive fitness (W)Amino acid substitution(s)
SH1000426.43 ± 0.991.00None
NR0061628.08 ± 1.241.00 ± 0.12PurR(G228E)
NR01712827.80 ± 1.911.12 ± 0.11PurR(V229G), NsaS(A105T)
NR01812827.69 ± 1.721.18 ± 0.15PurR(V229G), NsaS(A208E)
NR11112833.76 ± 3.021.02 ± 0.12PurR(E184STOP), MprF(S295L)