Table 4.

Reintroduction into S. aureus SH1000 of putative NISr mutations identified in strain NR018 to evaluate their contributions to NIS resistance

StrainNIS MIC (μg/ml)a
SH10004
NR018 [SH1000:PurR(V229G), NsaS(A208E)]b128
SH1000:PurR(V229G)c4
SH1000:NsaS(A208E)c32–64
SH1000:PurR(V229G), NsaS(A208E)c32–64
  • a MICs are determinations for a minimum of four independent experiments performed in triplicate.

  • b Strain generated by serial subculture in the presence of NIS.

  • c Strain generated by allelic replacement.