Table 2.

Percent decrease in the number of CFU per biofilm when 24-h-old S. aureus biofilms grown in different model systems were treated with VAN or CLI or a combination of an antibiotic and HAM

S. aureus strainTreatmentAvg % decrease ± SD in CFU obtained in different model systemsa
MHSCFMMLCWPBCWBRHE
CS1VAN2.2 ± 11.278.9 ± 7.82.0 ± 12.994.2 ± 1.584.1 ± 4.4
VAN-HAM68.4 ± 10.1*94.0 ± 3.0*95.0 ± 1.0*99.2 ± 0.2*99.1 ± 1.2*
CLI13.5 ± 9.568.3 ± 18.44.6 ± 11.274.9 ± 18.075.2 ± 2.9
CLI-HAM69.0 ± 8.8*97.8 ± 1.7*63.0 ± 8.8*96.8 ± 0.9*98.8 ± 1.0*
Mu50VAN2.6 ± 11.765.9 ± 6.74.4 ± 13.466.9 ± 32.491.2 ± 2.3
VAN-HAM71.3 ± 6.9*99.8 ± 0.1*82.4 ± 7.7*99.6 ± 1.7*99.6 ± 0.1*
CLI8.3 ± 17.48.4 ± 10.25.3 ± 11.26.8 ± 9.2ND
CLI-HAM6.2 ± 21.015.1 ± 12.212.1 ± 8.96.1 ± 8.5ND
  • a *, The results of combined treatment were significantly different from that of antibiotic treatment alone (P < 0.05). MH, Mueller-Hinton; SCFMM, synthetic cystic fibrosis medium with mucin; LCWPB, Lubbock chronic wound pathogenic biofilm; CWB, collagen wound biofilm; RHE: reconstituted human epithelium. ND, not determined.