Table 1.

Susceptibilities and molecular characteristics of NDM-1-producing E. coli CH01 and MH01

ParameterValue or characteristic for E. coli strain:
CH01MH01
MICa (μg/ml) of:
    AMC>16/8>16/8
    TZP>64/4>64/4
    FOX>16>16
    CRO>32>32
    CAZ>16>16
    ATM8>16
    MER>8>8
    IPM>8>8
    AMK>32>32
    GEN>8>8
    TOB>8>8
    CIP>2>2
    SXT>1/19>1/19
    NIT≤32>64
β-Lactamase(s)NDM-1NDM-1, CTX-M-15
Plasmid-mediated quinolone resistance determinantaac(6)-Ib-craac(6)-Ib
MLST resultST131ST101
Plasmid sizes (kb)120, 30075, 165, 300, 400
Replicon typing resultsIncA/C, IncFIAIncA/C, IncFII
Phylogenetic groupB2B1
Virulence characteristicsb
    Adhesins
        F10 papAPosNeg
        papACEFGNegNeg
        sfa focNegNeg
        focGNegNeg
        ihaPosNeg
        fimHPosPos
        tshNegNeg
        hraNegPos
        afa draNegNeg
    Toxins
        hlyDNegNeg
        satPosNeg
        picNegNeg
        vatNegNeg
        astANegNeg
        cnf1NegNeg
    Siderophores
        iroNNegNeg
        fyuAPosPos
        ireANegNeg
        iutAPosNeg
    Capsule
        kpsM IIPosNeg
        K1NegNeg
        K2PosNeg
        K5NegNeg
    Miscellaneous
        uspPosPos
        traTPosNeg
        ompTPosNeg
        issNegNeg
        fliCH7NegNeg
        malXPosNeg
  • a MICs were determined using the Microscan NEG MIC 30 panel. MIC values for the respective drugs in a drug combination are separated by a slash.

  • b The presence (Pos) or absence (Neg) of each virulence characteristic is indicated. F10 papA, P fimbria subunit variant; papACEFG, genes of P fimbria operon; sfa foc, S or F1C fimbriae; focG, F1C fimbria adhesin; iha, adhesion siderophore; fimH, type 1 fimbriae; tsh, temperature-sensitive hemagglutinin; hra, heat-resistant agglutinin; afa dra, Dr-binding adhesins; hlyD, α-hemolysin; sat, secreted autotransporter toxin; pic, serine protease; vat, vacuolating toxin; astA, enteroaggregative E. coli toxin; cnf1, cytotoxic necrotizing factor; iroN, salmochelin (siderophore) receptor; fyuA, yersiniabactin (siderophore) receptor; ireA, siderophore receptor; iutA, aerobactin (siderophore) receptor; kpsM II, group 2 capsule; K1, K2, and K5, group 2 capsule variants; usp, uropathogen-specific protein; traT, serum resistance-associated protein; ompT, outer membrane protease T; iss, increased serum survival; fliCH7, flagellin variant; malX, pathogenicity island marker.