Table 1.

Plasmids carrying blaKPC-2 genes in Enterobacteriaceae from Brazila

Inc groupSize (kb)bTn4401 variantOther beta-lactamasesSpeciesSTPFGE patterncSusceptibility phenotypedNo. of isolatesDateeCity/stateSample
FIIs50, 130, 240aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpAGEN3403/2007–06/2008Ribeirao Preto/SPSeveral samplesf
FIIs50, 130, 240aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpA1GEN106/2007Ribeirao Preto/SPSurgical wound
FIIs50, 130, 240aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpA2GEN202–06/2008Ribeirao Preto/SPBronco-alveolar lavage; urine
FIIs130, 150, 270aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpAGEN104/2007Franca/SPUrine
FIIs130aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpAGEN707–08/2009Ribeirao Preto/SPRectal swab
FIIs130aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpA1′GEN307–08/2009Ribeirao Preto/SPRectal swab
FIIs130aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpA4GEN208/2009Ribeirao Preto/SPRectal swab
FIIs130aOXA-9, TEM-1, SHV-1K. pneumoniaeST258KpA4′GEN108/2009Ribeirao Preto/SPRectal swab
FIIs130, 240aOXA-9, TEM-1, SHV-1K. pneumoniaeST11gKpA6GEN, AMI, TOB107/2009Ribeirao Preto/SPRectal swab
FIIs130, 140, 160aOXA-9, TEM-1, SHV-1, CTX-M-2K. pneumoniaeST48KpEAMI, CIP, LEV, NIT, STX108/2009Ribeirao Preto/SPRectal swab
ut50, 110bOXA-9, TEM-1, SHV-1, CTX-M-2K. pneumoniaeST44KpCGEN, AMI, TOB22008–2009Rio de Janeiro/RJUrine
N40, 100, 150bOXA-9, TEM-1, SHV-1, CTX-M-2K. pneumoniaeST327KpBGEN, AMI, TOB101/2008Rio de Janeiro/RJUrine
N40, 140bOXA-9, TEM-1, SHV-1, CTX-M-2K. pneumoniaeST437KpDGEN, AMI, TOB12009Rio de Janeiro/RJUrine
N40bOXA-9, TEM-1E. cloacaeNAEclαj211/2007–10/2008Porto Alegre; Lageado/RSFragment of foot amputated; urine
ut20, 100, 250, 300utOXA-9, TEM-1E. cloacaehNAEclβ301–04/2009Porto Alegre/RSVenous catheter; urine; blood
L/M60bOXA-9, TEM-1S. marcescensiNASmGEN104/2010Duque de Caxias/RJBlood
L/M50bOXA-9, TEM-1C. freundiiNACfAMI106/2010Duque de Caxias/RJBile secretion
  • a Abbreviations: GEN, gentamicin; AMI, amikacin; TOB, tobramycin; CIP, ciprofloxacin; LEV, levofloxacin; NIT, nitrofurantoin; STX, trimethoprim-sulfamethoxazole; NA, not applicable; ut = untypeable.

  • b Underlining indicates size of the plasmid carrying blaKPC-2.

  • c PFGE types are defined by capital letters. Subtypes are designated by a number (indicating the number of bands that differed from those of the index strain) and primes when necessary (to distinguish among subtypes differing in the same number of bands, with such bands being different).

  • d The following antibiotics were tested in this study: ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, cefazolin, cefuroxime, cefuroxime-axetil, cefotetan, ceftazidime, ceftriaxone, cefepime, aztreonan, imipenen, meropenen, AMI, GEN, TOB, CIP, LEV, NIT, STX, colistin, and tigecycline.

  • e Dates are given as month/year.

  • f This clone was recovered from samples of blood (n = 10), peritoneal cavity (n = 1), oropharynx (n = 2), urine (n = 7), venous catheter (n = 7), rectal swab (n = 3), broncoalveolar lavage (n = 1), sputum (n = 1), and wound/abscess (n = 2).

  • g ST11 and ST258 show highly related PFGE profiles, as has also been noted in other studies (37).

  • h Strains initially described in reference 40.

  • i Strain initially described in reference 11.

  • j —, susceptible only to colistin and tigecycline according to EUCAST breakpoints (13).