Table 6.

RT-qPCR analysis of the expression of pmrCAB in different strains compared to the level for the laboratory wild-type strain ATCC 17978a

StrainMean fold change ± SD
pmrCpmrApmrB
R2224.8 ± 140.651.7 ± 32.730.5 ± 18.5
R2 pmrB::Km1.43 ± 0.091.3 ± 0.4*
R2 pmrB::Km + pmrABWT1.0 ± 0.349.5 ± 8.1189.9 ± 17.0
R2 pmrB::Km + pmrABR2567.0 ± 137.058.8 ± 6.277.0 ± 9.2
R5165.2 ± 27.618.5 ± 0.410.6 ± 0.3
R5 pmrB::Km2.0 ± 1.20.9 ± 0.2*
C8292.1 ± 18.34.3 ± 0.052.7 ± 0.3
C8 pmrB::Km6.8 ± 0.61.7 ± 0.08*
C1426.5 ± 3.34.1 ± 0.73.2 ± 0.5
C14 pmrB::Km16.2 ± 1.82.9 ± 1.70.9 ± 0.1
  • a The values represent three independent samples, with each assessment being repeated in duplicate. *, no expression of pmrB could be detected in these strains.