Table 2.

MLST, spa types, and DNA microarray hybridization profiles of MRSA isolates M10/0061 and M10/0148 and MSSA isolate 21250_V454

Gene class and gene(s)DNA microarraya hybridization profile
M10/0061M10/014821250_V454
Species markers katA, coa, nuc, spaPosPosPos
agr group IIIPosPosPos
SCCmec-associated markers
    mecANegNegNeg
    ΔmecR1NegNegNeg
    mecR1-mecINegNegNeg
    ccrA1NegNegNeg
    ccrB1VariableVariableNeg
    Q9XB68-dcsNegNegNeg
    ccrA2-ccrB2NegNegNeg
    kdp-SCC locusNegNegNeg
    ccrA3VariableVariableNeg
    ccrB3VariableVariableNeg
    ccrC, ccrA4-ccrB4NegNegNeg
Antimicrobial resistance genes
    blaZNegNegNeg
    blaI-blaR1NegNegNeg
    erm(A), erm(B)NegNegNeg
    erm(C)NegNegNeg
    lnu(A)NegNegNeg
    msr(A), mph(C)NegNegNeg
    aacA-aphDNegNegNeg
    aadDNegNegNeg
    aphA3-satNegNegNeg
    dfrS1NegNegNeg
    far1NegNegNeg
    mupANegNegNeg
    tet(K)NegNegNeg
    tet(M)NegNegNeg
    catNegNegNeg
    cfrNegNegNeg
    fexANegNegNeg
    fosBNegNegNeg
    tet effluxPosPosPos
Virulence-associated genes
    tst1NegNegNeg
    sea, seb, see, sehNegNegNeg
    sec-sel, sed-sej-serNegNegNeg
    seg-sei-sem-sen-seo-seuNegNegNeg
    sek-seqNegNegNeg
    lukF-PV–lukS-PVNegNegNeg
    lukF-PV (P83), lukMNegNegPos
    hla, hldPosPosPos
    hlbPosPosPos
    Untruncated hlbPosPosPos
    sak-chp-scnNegNegNeg
    etA, etB, etDNegNegNeg
    edinA, edinBNegNegNeg
    edinBPosPosPos
    arcA-arcB-arcC-arcDNegNegNeg
Capsule type 8PosPosPos
  • a The StaphyType Kit (Alere Technologies) was used for DNA microarray analysis. The table shows only the clinically relevant markers, including exotoxins and resistance genes, and does not represent the complete hybridization profile of 334 probes on the array. Full data sets are available upon request. Isolate M10/0061 was CC130/ST130 and had the MLST allelic profile 6-57-45-2-7-58-52, spa type t843, and spa repeat succession 04-82-17-25-17-25-25-16-17. Isolate M10/0148 was CC130/ST1764 and had the MLST allelic profile 6-57-45-2-7-193-52, spa type t373, and spa repeat succession 04-17-25-17-25-16-17. Isolate 21250_V454 was CC130/ST2024 and had the MLST allelic profile 6-57-45-2-219-58-52, spa type t8403, and spa repeat succession 04-82-17-25-25-25-16-17. Pos, positive; Neg, negative; Variable, MRSA isolates M10/0061 and M10/0148 exhibited weak and/or variable signals for ccrB1, ccrA3, and ccrB3 in the primer-directed protocol but yielded positive signals for ccrB1 and ccrB3 in the random protocol outlined in the Materials and Methods.