Table 1

Description of strains and plasmids used in this study

StrainaRelevant genotype and phenotypeReference or source
S. aureus
    COLMethicillin-resistant SCCmec type I10
    N315Methicillin-resistant SCCmec type II29
    USA300Methicillin-resistant SCCmec type IV9, 27
    CK1001RN4220 ΔmprF::cat22
    KVRN315ΔvraSR::cat28
    RN4220Restriction-deficient mutagenized RN45015
    CB5011, pair ADS, SCCmec type II (USA100)Cubist Pharmaceuticals
    CB5012, pair ADR isogenic to CB5011; MprF L826FCubist Pharmaceuticals
    CB5013, pair BDS, SCCmec type II (USA100)Cubist Pharmaceuticals
    CB5014, pair BDR isogenic to CB5013, MprF S377LCubist Pharmaceuticals
    CB1631, pair CDS, SCCmec type II (USA100)Cubist Pharmaceuticals
    CB1634, pair CDR isogenic to CB1631, MprF L826FCubist Pharmaceuticals
    CB5048, pair DDS, SCCmec type IV (USA300)Cubist Pharmaceuticals
    CB5051, pair DDR isogenic to CB5048Cubist Pharmaceuticals
    CB5035, pair EDS, SCCmec type II (USA100)Cubist Pharmaceuticals
    CB5036, pair EDR isogenic to CB5035, MprF P314LCubist Pharmaceuticals
    CB182, pair FDS, SCCmec type IV (USA300)Cubist Pharmaceuticals
    CB183, pair FDR isogenic to CB182, MprF L341SCubist Pharmaceuticals
Derivative S. aureus strains
    MAR-1CB5011 ΔmprF::catThis study
    MAR-2MAR-1 + pMPRF-1 (wild-type mprF cloned into pPV72-2)This study
    MAR-3MAR-1 + pMPRF-2 (mutated mprF L826F cloned into pPV72-2)This study
    MAR-4CB5012+ ΔmprF::catThis study
    MAR-5MAR-4 + pMPRF-1This study
    MAR-6MAR-4 + pMPRF-2This study
    MAR-7CB5012 ΔvraSR::catThis study
    MAR-8MAR-7 + pVRASR-2This study
    MAR-9CB5011 expressing pVRASR-2This study
    MAR-10CB5013 expressing pVRASR-2This study
    MAR-11CB1631 expressing pVRASR-2This study
    MAR-12CB5048 expressing pVRASR-2This study
    MAR-13CB5035 expressing pVRASR-2This study
    MAR-14CB1631 ΔmprF::catThis study
    MAR-15MAR-14 pMPRF-1 (wild-type mprF cloned into pPV72-2)This study
    MAR-16MAR-14 pMRPF-2 (mutated mprF L826F cloned into pPV72-2)This study
    MAR-17CB1634 ΔmprF::catThis study
    MAR-18MAR-17(pMPRF-1) (wild-type mprF cloned into pPV72-2)This study
    MAR-19MAR-17(pMRPF-3) (mutated mprF L826F cloned into pPV72-2)This study
E. coli (PCR2.1-TOPO)Ampr KanrInvitrogen
S. aureus (pPV72-2)Shuttle vector; E. coli-S. aureus Tetr31
S. aureus RN4220 (pVRASR-2)Entire vraS/vraR cloned into pAW86
S. aureus RN4220 (pMPRF-1)Entire mprF cloned into pPV72-2This study
S. aureus RN4220 (pMPRF-2)Full-length mprF containing L826F site-directed mutation from CB5012 cloned into pPV72-2This study
S. aureus RN4220 (pMPRF-3)Full-length mprF containing L826F site-directed mutation from CB1634 cloned into pPV72-2This study
  • a Pair A, parent strain CB5011 and its derivative, CB5012; pair B, CB5013 and derivative CB5014; pair C, CB1631 and derivative CB1634; pair D, CB5048 and derivative CB5051; pair E, CB5035 and derivative CB5036; pair F, CB182 and derivative CB183.