Table 1

Primers used in this study

PrimerSequencea
SHV-1 Y105X
    Forward5′-GCAGGATCTGGTGGACNNSTCGCCGGTCAGCGAAA-3′
    Reverse5′-TTTCGCTGACCGGCGASNNGTCCACCAGATCCTGC-3′
Y105C
    Forward5′-GCAGGATCTGGTGGACTGCTCGCCGGTCAGCGAAA-3′
    Reverse5′-TTTCGCTGACCGGCGACGAGTCCACCAGATCCTGC-3′
Y105F
    Forward5′-GCAGGATCTGGTGGACTTCTCGCCGGTCAGCGAAA-3′
    Reverse5′-TTTCGCTGACCGGCGAGAAGTCCACCAGATCCTGC-3′
Y105I
    Forward5′-GCAGGATCTGGTGGACATCTCGCCGGTCAGCGAAA-3′
    Reverse5′-TTTCGCTGACCGGCGAGATGTCCACCAGATCCTGC-3′
Y105T
    Forward5′-GCAGGATCTGGTGGACACCTCGCCGGTCAGCGAAA-3′
    Reverse5′-TTTCGCTGACCGGCGAGGTGTCCACCAGATCCTGC-3′
  • a Boldface indicates site of codon substitution.