Table 5

Kds and inhibition constants for selected Y105 variantsa

SHVCefoperazone Kd (μM)Clavulanic acidPenem-1
kinh (s−1)Ki (μM)kinh (s−1)Ki (μM)
WT40 ± 40.022 ± 0.0051.1 ± 0.01ND0.040 ± 0.008
Y105E(2.8 ± 0.3) × 1030.11 ± 0.0259 ± 30.03 ± 0.020.9 ± 0.3
Y105F(1.3 ± 0.1) × 1020.11 ± 0.032.4 ± 0.4ND0.073 ± 0.009
Y105 M269 ± 30.13 ± 0.062.3 ± 0.50.15 ± 0.020.05 ± 0.02
Y105R(3.5 ± 0.4) × 1020.076 ± 0.0097.2 ± 0.8ND0.130 ± 0.003
  • a Determinations of kinetic parameters were performed in triplicate, and values are reported as means and standard deviations. ND, not determined.