Table 1

Kinetic parameters determined for Eis and acyl-CoA derivatives with various AGs

CoA analogAGValue (mean ± SD) of:
Km (μM)kcat (min−1)kcat/Km (M−1 s−1)
Acetyl-CoAAMK7.88 ± 1.351.14 ± 0.302,411 ± 757
HYG176.62 ± 6.4216.26 ± 0.481,534 ± 72
KAN9.44 ± 1.205.56 ± 0.369,816 ± 1,400
NEA47.30 ± 1.464.50 ± 0.361,586 ± 136
NEO24.44 ± 0.686.54 ± 0.184,460 ± 175
NET62.95 ± 2.4414.82 ± 1.023,923 ± 310
PAR45.50 ± 5.523.24 ± 0.781,187 ± 320
RIB22.18 ± 1.723.54 ± 0.062,660 ± 211
SIS69.41 ± 0.804.20 ± 0.121,009 ± 31
TOB47.04 ± 1.3116.92 ± 4.925,995 ± 1,751
n-Propionyl-CoAAMK53.14 ± 6.181.14 ± 0.30358 ± 103
HYG30.84 ± 0.621.80 ± 0.01973 ± 20
KAN55.66 ± 13.280.48 ± 0.06144 ± 39
NEA55.53 ± 6.080.60 ± 0.06180 ± 28
NEO53.22 ± 0.141.98 ± 0.12620 ± 38
NET31.24 ± 3.763.23 ± 0.021,723 ± 208
PAR25.12 ± 2.620.48 ± 0.06318 ± 52
RIB66.76 ± 3.611.32 ± 0.30330 ± 77
SIS105.79 ± 4.796.42 ± 1.501,011 ± 241
TOB74.49 ± 7.786.36 ± 0.241,423 ± 158
Crotonyl-CoANEO88.86 ± 7.400.33 ± 0.0462 ± 9
Malonyl-CoANEO81.49 ± 5.071.51 ± 0.02309 ± 20