Table 3

MICs of standard antibiotics and PPMOs using transposome mutant RR3, isogenic parent strain XL1-Blue MRF′, and their sbmA complemented strains

PPMO no.Antibiotic or PPMOMIC (μM [μg/ml])
XL1-Blue MRF′RR3XL1-Blue MRF′ (pSbmA)RR3(pSbmA)
Polymyxin B0.8 (1)0.8 (1)0.8 (1)0.8 (1)
Colistin0.9 (1)0.9 (1)0.9 (1)0.9 (1)
Erythromycin34 (25)34 (25)34 (25)34 (25)
Rifampin6 (5)6 (5)6 (5)6 (5)
Bleomycin0.2 (0.25)0.7 (1)0.01 (0.016)0.01 (0.016)
Phleomycin0.3 (0.5)1.3 (2)0.02 (0.03)0.02 (0.03)
NG-05-0200(RFF)3RXB-AcpP2 (11)64 (355)2 (11)2 (11)
NG-05-0653[d-(RFF)3R]XB-AcpP2 (11)64 (355)2 (11)2 (11)
NG-06-0076(RXR)4XB-AcpP1 (6)8 (48)0.1 (0.6)0.1 (0.6)
NG-05-0655(RFF)3RXB-Scr>128 (>714)>128 (>714)>128 (>714)>128 (>714)
NG-06-0078(RXR)4XB-Scr>128 (>714)>128 (>714)>128 (>714)>128 (>714)