Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or characteristicsbReference
    P. aeruginosa
        WTWild-type P. aeruginosa PAO1 strain H10337
        UW-PA3077aPA3077::ISlacZ/hah-Tcr; insertion at bp 502 (672) in PA3077; derived from UW-WT18
        UW-PA3078PA3078::ISpho/hah-Tcr; insertion at bp 739 (1296) in PA3078; derived from UW-WT18
        cprRPA3077::ISlacZ/hah-Tcr, H103 background; TcrThis study
        cprSPA3078::ISpho/hah-Tcr, H103 background; TcrThis study
        cprR+cprR mutant with Tn7-cprRS+ integrated; Tcr GmrThis study
        cprS+cprS mutant with Tn7-cprRS+ integrated; Tcr GmrThis study
        parRPA1799::ISlacZ/hah-Tcr, H103 background; Tcr10
    E. coli TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
    pCR-Blunt II-TOPOPCR cloning vector; KanrInvitrogen
    pCR-cprRS+pCR-Blunt II-TOPO harboring 2.5-kb cprRS ampliconThis study
    pUC18-mini-Tn7T-GmSuicide plasmid; Gmr Ampr5
    pUC-Tn7-cprRS+pUC18-mini-Tn7T-Gm with 2.5-kb cprRS fragment from pCR-cprRSThis study
    pTNS2Transposition helper plasmid; Ampr5
    pUCPlux-PPA3552pUCP23 containing the entire intergenic region between PA3551 and PA3552 fused to luxCDABE25
  • a UW, University of Washington Genome Science Center.

  • b Antibiotic resistance phenotypes: Ampr, ampicillin for E. coli and carbenicillin for P. aeruginosa; Gmr, gentamicin; Kanr, kanamycin; Tcr, tetracycline; Strr, streptomycin.